Patrick Astarita
6 days ago
Question

Building a Biomanufacturing Knowledge-base

Building a Biomanufacturing Knowledge-base

Let's chat Feedstocks, Cellular Chassis, Fermentation Systems, DSP, and Packaging. Who's trying to manufacturing commodity-competitive products with microbes in vats? I'm open to sharing my experience and expertise in designing & building consumer hardware, electronic devices, chemical processing equipment, liquid handlin...
Sebastián Sosa Carrillo
18 days ago
Method

Quick and easy bioengineering procedures: Yeast (S. cerevisiae) colony PCR

Quick and easy bioengineering procedures: Yeast (S. cerevisiae) colony PCR

Hi all, This is the last post in a series of 4 in which I want to share a quick and easy to use procedure for engineering a yeast strain. The idea is to go from the cloning stage, right after doing a PCR (or order) to get the DNA you want to clone, up to the process of transforming the yeast and selecting the positive clon...
Ruben Ramalho
20 days ago
Pro Lab Tip

The unreasonable effectiveness of 3D printers

Guy Rohkin
22 days ago

AMA Recording with Dr. Phillip Kyriakakis. Flipping the Molecular Light Switch: Dr. Phillip Kyriakakis and the World of Optogenetics

AMA Recording with Dr. Phillip Kyriakakis. Flipping the Molecular Light Switch: Dr. Phillip Kyriakakis and the World of Optogenetics

Hey all! Posting the recording here to Dr. Phillip Kyriakakis' (https://scifind.io/profiles/phillip-kyriakakis-i73l2rqgm6dfxlu) AMA! Really fun chat and great to hear about all his work and journey. (https://the-fancy-labcoat-guild.simplecast.com/episodes/flipping-the-molecular-light-switch-dr-phillip-kyriakakis-and-the-...
Optogenetics Genetic EngineeringSynthetic BiologyHardware Electrical Engineering
Karissa Milbury
23 days ago
Method

Dilution spot assay for yeast

Dilution spot assay for yeast

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...
Karissa Milbury
about 1 month ago
Method

"Quick and dirty" S. cerevisiae gDNA prep protocol

"Quick and dirty" S. cerevisiae gDNA prep protocol

This protocol served me well through my PhD and postdoc years. Dilute the final product at least 1/10 for use in PCR. Spin down 50-100 uL packed volume of yeast cells Resuspend in 200 uL TE + 1% SDS and move the cells to a 1.5ml screw-top tube if possible Add 200 uL of glass beads (eyeball this: just pour in beads until th...
Guy Rohkin
about 1 month ago

The Genetic Underground Rave on May 24th in Oakland, CA

Guy Rohkin
about 2 months ago

April 20th, 11am PDT/2pm PST Discord chat. Flipping the Switch: An AMA with the “Illuminating” Dr. Phillip Kyriakakis

April 20th, 11am PDT/2pm PST Discord chat. Flipping the Switch: An AMA with the “Illuminating” Dr. Phillip Kyriakakis

🧪 Get ready for an enlightening experience on optogenetics–in short, molecular switches for regulating light-controlled expression! Join us on April 20th at 11am PDT in “The Salon” Discord channel (https://discord.gg/s7E6nDxDEn?event=1097203992959922198) as we welcome Dr. Phillip Kyriakakis, Senior Research Scientist at St...
Efi Kontou
about 2 months ago
MethodPaper discussion

How to claim that a compound is produced by your strain in a publication?

Efi Kontou
about 2 months ago
Method

Useful tutorials for mass spectrometry

Useful tutorials for mass spectrometry

Hi everyone! Posting the seminars hosted by the Functional Metabolomics Lab (Daniel Petras) in Tuebingen, Germany, about mass spectrometry (intro theory to specific workflows for analyzing your data). They are super useful for people that have metabolomics/proteomics data and are newbies in the field! https://youtube.com/pl...
Mass Spectrometry Proteomics Metabolomics Synthetic Biology