Giuseppe D'Agostino
14 days ago
Method

Is your UMAP lying to you?

Is your UMAP lying to you?

Intro: to see is to know Humans are very visual creatures, and the way we experience science - especially biological science - is predominantly through visualizations. From beautiful immunofluorescence stainings to western blot bands, going through action potential recordings, scientists look at data before anything else....
Single Cell Genomics data visualization UMAP dimensionality reduction Bioinformatics Computational method Rstats
Di Hu
9 months ago

FACS purification of target nuclei population for 10x multiome of human brain and spinal cord

FACS purification of target nuclei population for 10x multiome of human brain and spinal cord

The 10x multiome kit allows you to link open chromatin (ATAC) and gene expression at single cell resolution https://www.10xgenomics.com/products/single-cell-multiome-atac-plus-gene-expression?https%3A%2F%2Fwww.10xgenomics.com%2F%3Fsrc=search&utmmedium=search&utmsource=google&utmcampaign=sem-goog-2022-website-page-rag-pscmag...
GenomicsNeuroscienceSingle Cell Genomics single-cell transcriptomes single-cell sorting single-cell RNA sequencing Genomic DNA Library PreparationNGS Library PreparationSequencingFACSFACS StainingBrainSpinal CordAntibody DetectionHumans
Di Hu
9 months ago

Open platform for accurate >95% recovery single cell -omics: cellenONE

Open platform for accurate >95% recovery single cell -omics: cellenONE

Use the cellenONE https://www.cellenion.com/products/cellenone-x1/ if you'd like an open platform for the single cell -omics of your choice (e.g. I used it to develop new single cell epigenetic and multiomic profiling methods). It has: nanoliter dispensing so you can save on reagents temperature/dew point controlled inter...
Single Cell Isolation single-cell sorting single-cell RNA sequencing EpigeneticsGenomic DNA Library PreparationNGS Library PreparationSequencingCancerHealthy AgingGenomicsRoboticsSingle cell cloningSingle Cell Genomics
Di Hu
9 months ago

Trick to capturing gene expression and epigenetics from the same cell – it's not rocket science, it's multiomics

Trick to capturing gene expression and epigenetics from the same cell – it's not rocket science, it's multiomics

Simultaneous Profiling of Gene Expression and Chromatin Accessibility in Single Cells ATAC-RNA Joint Profiling Workflow Though this could work for any type of gDNA library, e.g. CUT&Tag for profiling histone marks and TF binding) Sorting and DSP Fixation Sort 50,000-100,000 cells into RPMI + 10% FBS Spin down 300g 5 min...
GenomicsTranscriptomicsEpigenomicsSequencingSingle Cell GenomicsGenomic DNA Library PreparationNGS Library Preparation qPCR FACSBioanalyzerRNA-sequencing