Michael Paulsmeyer
9 months ago
Method

Best Practices for PCR Primer Design

Best Practices for PCR Primer Design

Intro PCR is definitely one of those things in science that borders on magic and witchcraft. I like to think I know what I am doing, but I still have those days even in my postdoc that I have to pray to the PCR Gods to get something to work. In my experience, PCR success is mainly dependent on the primer set that you use....