Plasmid Extraction

3 months ago

This is my go-to alakline lysis miniprep protocol that does not require a kit or silica columns. It's based on the tried and true protocols of yesteryear and brought to my attention a few years ago by J. Leon (@jesussaur) on Twitter. I've been using it ever since and have successfully sent plasmids out for sequencing via pl...

8 months ago

First of all, @Michael Paulsmeyer had a great post a while back on how to get as much DNA from a standard miniprep as possible (https://www.scifind.net/methods/efficient-miniprep-spin-column-k). The variance in my personal results from minipreps is large, though. To make sure I always get a high concentration of DNA, I use ...

9 months ago

Here is my workflow for high plasmid yields from cultures of DH5α E. coli. I use the GeneJet Plasmid Miniprep kit from Thermo (#K0502), but most spin column kits work the same. Generally I have to extract plasmids from numerous cultures simultaneously, so this protocol also gives guidance on the timing of each step. Prick ...

Plasmid Extraction

Columnless Old-School Miniprep

Columnless Old-School Miniprep

Miraprep: >30μg of Plasmid DNA Using a Miniprep Kit Every Time

Miraprep: >30μg of Plasmid DNA Using a Miniprep Kit Every Time

Efficient miniprep spin-column kit workflow for high plasmid yields

Efficient miniprep spin-column kit workflow for high plasmid yields