Karissa Milbury
21 days ago
Method

Dilution spot assay for yeast

Dilution spot assay for yeast

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...
Karissa Milbury
about 1 month ago
Method

"Quick and dirty" S. cerevisiae gDNA prep protocol

"Quick and dirty" S. cerevisiae gDNA prep protocol

This protocol served me well through my PhD and postdoc years. Dilute the final product at least 1/10 for use in PCR. Spin down 50-100 uL packed volume of yeast cells Resuspend in 200 uL TE + 1% SDS and move the cells to a 1.5ml screw-top tube if possible Add 200 uL of glass beads (eyeball this: just pour in beads until th...
Peter Sobolewski
8 months ago

Open Hardware: Sidekick: A Low-Cost Open-Source 3D-printed liquid dispensing robot