J G
about 2 hours ago
QuestionMethod

Bacterial allelic exchange - chromosomal integration problem

Ayşe Sunar
6 days ago
QuestionMethod

List of Protein Kinase Pseudogenes?

Sebastián Sosa Carrillo
18 days ago
Method

Quick and easy bioengineering procedures: Yeast (S. cerevisiae) colony PCR

Quick and easy bioengineering procedures: Yeast (S. cerevisiae) colony PCR

Hi all, This is the last post in a series of 4 in which I want to share a quick and easy to use procedure for engineering a yeast strain. The idea is to go from the cloning stage, right after doing a PCR (or order) to get the DNA you want to clone, up to the process of transforming the yeast and selecting the positive clon...
Guy Rohkin
about 1 month ago

The Genetic Underground Rave on May 24th in Oakland, CA

Guy Rohkin
about 2 months ago

April 20th, 11am PDT/2pm PST Discord chat. Flipping the Switch: An AMA with the “Illuminating” Dr. Phillip Kyriakakis

April 20th, 11am PDT/2pm PST Discord chat. Flipping the Switch: An AMA with the “Illuminating” Dr. Phillip Kyriakakis

🧪 Get ready for an enlightening experience on optogenetics–in short, molecular switches for regulating light-controlled expression! Join us on April 20th at 11am PDT in “The Salon” Discord channel (https://discord.gg/s7E6nDxDEn?event=1097203992959922198) as we welcome Dr. Phillip Kyriakakis, Senior Research Scientist at St...
Thomas Schmitt
about 2 months ago
Question

Study on Partial Glycoprotein of Feline Herpesvirus Type 1

Adán Andrés Ramírez Rojas
2 months ago
Pro Lab Tip

"Mid/High-throughput" transformation

Sebastian Cocioba
2 months ago
Method

Ultra-cheap PCR Precipitation using PEG and Salt

Jessie MacAlpine
8 months ago

Two New Papers on Pan-Cancer Mycobiota Profiles in Cell

Nick Gervais
8 months ago

Resuspending Twist Oligo Pools?

Resuspending Twist Oligo Pools?

Hi everyone, I've received a custom oligo pool from Twist Bioscience and they provided 70ng of it. We talked to one of the reps and they said not to resuspend it to any less than 10ng/μL, which means I'll be adding less than 7μL of buffer, which is obviously not a lot. I was wondering if anyone had any practical tips for h...
Genetic EngineeringMolecular BiologyGeneticsMicrobiology Twist Oligo Pools Twist Bioscience Oligo Pool