Karissa Milbury
21 days ago
Method

Dilution spot assay for yeast

Dilution spot assay for yeast

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...
Henrik Ellinghaus
8 months ago
Method

Western Blot

Western Blot

Who has not yet had "fun" with Western Blots? This technique to identify specific proteins in a solution is widely used in laboratories world wide. If you have experience with it you know how desperate it can be... To better understand the basic method and to see at which points one can troubleshoot, I created an animated ...