Efi Kontou
about 2 months ago
MethodPaper discussion

How to claim that a compound is produced by your strain in a publication?

Efi Kontou
about 2 months ago
Method

Useful tutorials for mass spectrometry

Useful tutorials for mass spectrometry

Hi everyone! Posting the seminars hosted by the Functional Metabolomics Lab (Daniel Petras) in Tuebingen, Germany, about mass spectrometry (intro theory to specific workflows for analyzing your data). They are super useful for people that have metabolomics/proteomics data and are newbies in the field! https://youtube.com/pl...
Mass Spectrometry Proteomics Metabolomics Synthetic Biology
Efi Kontou
2 months ago
Method

Preparing micro-scale fermentations of bacteria/fungi for untargeted metabolomics analysis

Preparing micro-scale fermentations of bacteria/fungi for untargeted metabolomics analysis

Here is the protocol I used for sample preparation of small-scale cultivations for untargeted metabolomics studies: Note that no sample preparation method is perfect, and at every step of the way you might lose compounds 1) Grow the bacteria/fungi in 4x24-wells using 3.14 mL of media + spore inoculum (O.D. = 0.1) + 5 glas...
Synthetic Biology Untargeted metabolomics LC-MSMass SpectrometrySample Preparation Positive pressure manifold Mass Spectrometers LC-MS Nitrogen stream
Amos Fung
almost 2 years ago
Method

MALDI-TOF Protein Prep

MALDI-TOF Protein Prep

Mass spectrometry is a commonly identification method for the products of a protein digest, modification, purification, or the like. Sample preparation is handled via Ziptips, an extremely fast (if not expensive) method of reducing contaminants in your sample. ZipTipping solutions A. 0.2% TFA in HPLC grade water; for dil...