Pearl Satyr
10 months ago

Gene knockout protocol from HCC cancer cell lines using px459-v2?

Blonde Centaur
over 1 year ago

Cas13 RNA design for knockdown of chicken cells

Tan Naga
about 2 years ago

question about SAM

Snow Gremlin
about 2 years ago

Stable Cas9 expressing hESCs H1

Stable Cas9 expressing hESCs H1

I am in the process of developing a CRISPR screen which requires Cas9 expressing hESCs. I have been following the protocol outlined in the Joung et al. 2017 Nature protocols paper. However, I am having a lot of difficulty producing a Cas9 expressing H1 cell line. I have transduced these cells in parallel with a GFP lentivir...
Coral Griffin
over 2 years ago

dCas9-VP64 Lenti System

Peach Manticore
over 2 years ago

nucleofection with Lonza of suspension cells- low efficiency with 10 kb plasmid

Yellow Pixie
over 2 years ago

DNA is edited but protein still remains, is this a problem?

Turquoise Ghoul
over 2 years ago

CRISPR Gecko v2 library with lentiviral ready.

Ivory Kraken
over 2 years ago

Basic question about sgRNA designing

Terracotta Wendigo
over 2 years ago

CRISPR library transduced cell numbers I need to keep during passage after puromycin selection