Patrick Astarita
4 days ago
Question

Building a Biomanufacturing Knowledge-base

Building a Biomanufacturing Knowledge-base

Let's chat Feedstocks, Cellular Chassis, Fermentation Systems, DSP, and Packaging. Who's trying to manufacturing commodity-competitive products with microbes in vats? I'm open to sharing my experience and expertise in designing & building consumer hardware, electronic devices, chemical processing equipment, liquid handlin...
Karissa Milbury
about 1 month ago
Method

"Quick and dirty" S. cerevisiae gDNA prep protocol

"Quick and dirty" S. cerevisiae gDNA prep protocol

This protocol served me well through my PhD and postdoc years. Dilute the final product at least 1/10 for use in PCR. Spin down 50-100 uL packed volume of yeast cells Resuspend in 200 uL TE + 1% SDS and move the cells to a 1.5ml screw-top tube if possible Add 200 uL of glass beads (eyeball this: just pour in beads until th...
Sebastian Cocioba
3 months ago
Pro Lab Tip

NaDCC Bleach for Surface Sterilization of Seeds and Plant Tissues

NaDCC Bleach for Surface Sterilization of Seeds and Plant Tissues

I found this handy bleach called NaDCC (Sodium dichloroisocyanurate), commonly used as water purification tablets. The brand I use is ef-chlor (167mg) and I make stock solutions by dissolving one tablet into 50mL of distilled water (2000ppm of active chlorine) and then diluting to 500ppm to make a working stock. I typically...
Plant Tissue CultureTissue Culture TechniquesLaboratory Shakers Surface Sterilization Brassica rapa Nicotiana tabacum
Amos Fung
almost 2 years ago
Method

IPTG Induction for Protein Expression