Genetics

5 days ago

Hello everyone! I have just been made aware of this community and am very glad to finding it! So here is my question: I'm working with a protein kinase dataset for my prject however I need to remove the protein kinase pseudogenes from the dataset. Is there a dataset where I could find the list of all of the protein kinase...

21 days ago

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...

4 months ago

This is a method for low-cost electrotransformation of electrocompetent NEB turbo E coli cells using a homemade electroporation apparatus. The original paper upon which this method is based can be found here: https://doi.org/10.1371/journal.pbio.3000589 My method for preparing electrocompetent E coli can be found here: h...

8 months ago

Hi everyone, I've received a custom oligo pool from Twist Bioscience and they provided 70ng of it. We talked to one of the reps and they said not to resuspend it to any less than 10ng/μL, which means I'll be adding less than 7μL of buffer, which is obviously not a lot. I was wondering if anyone had any practical tips for h...

8 months ago

First of all, @Michael Paulsmeyer had a great post a while back on how to get as much DNA from a standard miniprep as possible (https://www.scifind.net/methods/efficient-miniprep-spin-column-k). The variance in my personal results from minipreps is large, though. To make sure I always get a high concentration of DNA, I use ...

9 months ago

Does the transgene from lentiviral transduction integrate in a location in the genome specific to the sequence of transfer vector or does it depend on the cell? Like, given the scenario that I wanted to attempt a double knockdown with the same transfer vector and expand clones to characterize ones with both inserts - if th...

10 months ago

So, let’s talk ethics and gene editing. Designer dogs have been around as long as domestication… We know where that went- ever seen a pug? “Cute” brachycephalic dogs with severe health issues and short lifespans, just for fun. Now, we’ve got CRIPSR because, obviously, we can’t spend generations and generations in-breeding ...

List of Protein Kinase Pseudogenes?

List of Protein Kinase Pseudogenes?

Dilution spot assay for yeast

Dilution spot assay for yeast

"DIY" Electroporation of NEB Turbo Using "Electropen"

"DIY" Electroporation of NEB Turbo Using "Electropen"

Resuspending Twist Oligo Pools?

Resuspending Twist Oligo Pools?

Miraprep: >30μg of Plasmid DNA Using a Miniprep Kit Every Time

Miraprep: >30μg of Plasmid DNA Using a Miniprep Kit Every Time

Question about Lentiviral Transduction

Question about Lentiviral Transduction

Designer Babies

Designer Babies