Karissa Milbury
21 days ago
Method

Dilution spot assay for yeast

Dilution spot assay for yeast

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...
Michael Paulsmeyer
9 months ago
Method

Efficient miniprep spin-column kit workflow for high plasmid yields

Efficient miniprep spin-column kit workflow for high plasmid yields

Here is my workflow for high plasmid yields from cultures of DH5α E. coli. I use the GeneJet Plasmid Miniprep kit from Thermo (#K0502), but most spin column kits work the same. Generally I have to extract plasmids from numerous cultures simultaneously, so this protocol also gives guidance on the timing of each step. Prick ...