Adapter trimming in 10x Data and CellRanger Make Counts Expected Cells

_._darachm_._ {LastName}
8 days ago
MethodPro Lab Tip

Warnings, solutions, and mysteries regarding extraction/PCR from large amounts of yeast stationary-phase genomes

Warnings, solutions, and mysteries regarding extraction/PCR from large amounts of yeast stationary-phase genomes

This post is about some perplexing and difficult to get rid of contaminant that accumulates in yeast, especially stationary phase samples, that inhibits PCRs in a hard to predict way. Now I'm no biochemist, so I don't have a solution for you, but I do have a dirty hack that should let you do large batches of extracts that a...
Anan Tarabeih
about 2 months ago
Method

CUT & TAG Kit - Tn5

Guy Rohkin
10 months ago
Method

Using the Illumina Nextera XT kit at 1/3 reagents

Using the Illumina Nextera XT kit at 1/3 reagents

In a genomics lab, if you need to work with really low input DNA samples you can use the Illumina Nextera XT kit. That being said, the kit is rather expensive and can be adapted to work perfectly fine by using 1/3 reagents for your reactions! You'll get pretty much the same results just make sure you keep the ratio of volum...