Karissa Milbury
about 1 month ago
Method

"Quick and dirty" S. cerevisiae gDNA prep protocol

"Quick and dirty" S. cerevisiae gDNA prep protocol

This protocol served me well through my PhD and postdoc years. Dilute the final product at least 1/10 for use in PCR. Spin down 50-100 uL packed volume of yeast cells Resuspend in 200 uL TE + 1% SDS and move the cells to a 1.5ml screw-top tube if possible Add 200 uL of glass beads (eyeball this: just pour in beads until th...
Sebastian Cocioba
2 months ago
Method

Ultra-cheap PCR Precipitation using PEG and Salt

Adhirath Sikand
over 1 year ago
Method

small DNA/oligos Circularization and purification

small DNA/oligos Circularization and purification

DNA oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA) and their sequences. All oligomers were purified in-house using denaturing PAGE purification. The templates contained a 5ā€™ phosphate modification in order to circularize the template. Briefly, the template to be circularized was annea...
BiochemistryDNA Purification DNA circularization