Di Hu
9 months ago

SPRI bead size selection guidance for histone marks

SPRI bead size selection guidance for histone marks

You've prepared your epigenetic library for profiling histone marks. If you are not using the Zymo ChIP DNA Clean & Concentrator Kit for final elution, you may want to size select for your library region of interest using SPRI bead clean up. How much bead do you add relative to your volume of library? Because adapters for ...
GenomicsEpigeneticsNGS Library PreparationSequencingMagnetic BeadsGenomic DNA Library PreparationEpigenomicsQuality AssessmentSample PreparationDNA library preparation
Di Hu
9 months ago

CUT&Tag is a recent method to profile epigenetic marks with reduced cell numbers and cost, but how does it compare to ChIP-seq?

CUT&Tag is a recent method to profile epigenetic marks with reduced cell numbers and cost, but how does it compare to ChIP-seq?

Benchmarking CUT&Tag against ChIP-seq: https://www.biorxiv.org/content/10.1101/2022.03.30.486382v1 https://www.nature.com/articles/s41467-019-09982-5 Lovely blog post on CUT&Tag: https://www.activemotif.com/blog-cut-tag The improved sensitivity of CUT&Tag compared to ChIP-seq is due to the use of pA-Tn5 to streamline lib...
GenomicsChIP-SequencingChromatinEpigenomicsEpigeneticsSequencingGenomic DNA Library PreparationDNA library preparationBenchmarkingDNA AmplificationPCRNext Generation Sequencing