Henrik Ellinghaus
8 months ago
Method

PCR (Polymerase Chain Reaction)

PCR (Polymerase Chain Reaction)

PCR is likely one of the most useful techniques in biology labs. It was invented by Kary Mullis in 1984. Nowadays it helps us especially if DNA material needs to be amplified to work with higher quantities, but the applications of this method are almost endless. Each PCR protocol uses the same standard ingredients. The pro...
PCRDNADNA Amplification
Di Hu
9 months ago

CUT&Tag is a recent method to profile epigenetic marks with reduced cell numbers and cost, but how does it compare to ChIP-seq?

CUT&Tag is a recent method to profile epigenetic marks with reduced cell numbers and cost, but how does it compare to ChIP-seq?

Benchmarking CUT&Tag against ChIP-seq: https://www.biorxiv.org/content/10.1101/2022.03.30.486382v1 https://www.nature.com/articles/s41467-019-09982-5 Lovely blog post on CUT&Tag: https://www.activemotif.com/blog-cut-tag The improved sensitivity of CUT&Tag compared to ChIP-seq is due to the use of pA-Tn5 to streamline lib...
GenomicsChIP-SequencingChromatinEpigenomicsEpigeneticsSequencingGenomic DNA Library PreparationDNA library preparationBenchmarkingDNA AmplificationPCRNext Generation Sequencing