DNA

8 months ago

PCR is likely one of the most useful techniques in biology labs. It was invented by Kary Mullis in 1984. Nowadays it helps us especially if DNA material needs to be amplified to work with higher quantities, but the applications of this method are almost endless. Each PCR protocol uses the same standard ingredients. The pro...

8 months ago

First of all, @Michael Paulsmeyer had a great post a while back on how to get as much DNA from a standard miniprep as possible (https://www.scifind.net/methods/efficient-miniprep-spin-column-k). The variance in my personal results from minipreps is large, though. To make sure I always get a high concentration of DNA, I use ...

PCR (Polymerase Chain Reaction)

PCR (Polymerase Chain Reaction)

Miraprep: >30μg of Plasmid DNA Using a Miniprep Kit Every Time

Miraprep: >30μg of Plasmid DNA Using a Miniprep Kit Every Time