Sebastián Sosa Carrillo
17 days ago
Method

Quick and easy bioengineering procedures: Yeast (S. cerevisiae) colony PCR

Quick and easy bioengineering procedures: Yeast (S. cerevisiae) colony PCR

Hi all, This is the last post in a series of 4 in which I want to share a quick and easy to use procedure for engineering a yeast strain. The idea is to go from the cloning stage, right after doing a PCR (or order) to get the DNA you want to clone, up to the process of transforming the yeast and selecting the positive clon...
Sebastián Sosa Carrillo
about 2 months ago
Method

Quick and easy bioengineering procedures: Yeast (S. cerevisiae) transformation

Adán Andrés Ramírez Rojas
2 months ago
Pro Lab Tip

"Mid/High-throughput" transformation

Sebastián Sosa Carrillo
2 months ago
Method

Quick and easy bioengineering procedures: Competent cells and transformation

Sebastián Sosa Carrillo
2 months ago
Method

Quick and easy bioengineering procedures: Golden gate cloning

Maddie Watts
3 months ago
Question

GoTaq G2 polymerase vs high-fidelity in CPEC cloning?

Amos Fung
almost 2 years ago
Method

Higher Yield Gel Extractions

Amos Fung
almost 2 years ago
Method

Colony PCR

Colony PCR

PCR can be performed directly from a scraped cell colony, using an extended denaturing step to cause lysis of the cells. This is a nice time saving protocol for PCRing targets directly from antibiotic selective plate media. Colony PCR with Patch Plating If subculturing via patch plate, divide a warm LB-Amp plate into a gri...
Amos Fung
almost 2 years ago
Method

X-Gal Blue White Screening

Amos Fung
almost 2 years ago
Method

TOPO TA Cloning