Julian West
9 months ago

Removing Toxic Biomolecules that Accumulate with Age

Removing Toxic Biomolecules that Accumulate with Age

Something I love about scientific conferences is being exposed to less widely known - but potentially very important - avenues of research. Case in point: the accumulation of a toxic biomolecule, an oxidized form of cholesterol (7-ketocholesterol : https://pubchem.ncbi.nlm.nih.gov/compound/7-Ketocholesterol ), believed to ...
LongevityCell BiologyPharmacologyCholesterol Atherosclerosis Cyclodextrins 7-ketocholesterol Cyclarity Therapeutics Matthew O'Connor Conferences Ending Age-Related Diseases 2022 UDP-003 Candymer software Healthspan
Sam
about 1 year ago
Method

Materials and Methods

Materials and Methods

Materials All reagents were ordered from Sigma-Aldrich (St. Louis, MO, USA) unless specified otherwise. Linear PEI (molecular weight, 40 kDa) was obtained from Polysciences Inc. (Warrington, PA, USA). U2OS and 3T3-L1 were obtained from the American Type Culture Collection (ATCC). U2OS.EGFP reporter cell line was obtained f...