Mandy Lorewall
15 days ago
Question

Cell Culture Question:

Karissa Milbury
21 days ago
Method

Dilution spot assay for yeast

Dilution spot assay for yeast

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...
Anan Tarabeih
about 2 months ago
Method

CUT & TAG Kit - Tn5

Efi Kontou
2 months ago
Pro Lab TipMethod

Scaling down fermentations of Actinomycetes

Scaling down fermentations of Actinomycetes

Actinomycetes are known to be difficult to grow in micro-scale fermentations due to the aggregation of their pellets into "clumps", a common issue that leads to a lack of oxygenation in such a small space as a 96 or 24-well plate (suffocation if you will). So scientists are often afraid to scale down their fermentations, in...
Actinomycetes 24-well plates glass beads BacteriaCell CultureSynthetic Biology Small-scale
Peter Sobolewski
7 months ago

Improving cell seeding in well plates using a Uniform Cell Seeding (UCS) 3D printed insert

Improving cell seeding in well plates using a Uniform Cell Seeding (UCS) 3D printed insert

When doing cell adhesion studies, we want to know what percentage of the seeded cells actually adhered to the material (for example a thin, spin coated film on a coverslip). We can image the coverslip and using the area and cell density calculate this percentage. However, if the cells are not seeded uniformly, this can aff...
Henrik Ellinghaus
7 months ago
Method

Manual Cell Counting with Counting Chambers (video guide)

Manual Cell Counting with Counting Chambers (video guide)

Dear Scientists, a while ago I made a video on how to count cells manually with counting chambers (e.g. Neubauer counting chamber). This video guide breaks down each step and explains how you can determine the number of cells in your solution with ease! Please check it out if you need to work with the Counting chambers an...
Cell Culture cell counting neubauer counting chamber
Henrik Ellinghaus
7 months ago
Method

Clonogenic Assay (Colony Formation Assay)

Clonogenic Assay (Colony Formation Assay)

One way to determine the effect of treatments (e.g. drugs) on cell proliferation and survival is to work with the Colony Formation Assay. I have prepared a video guide on how to perform this assay: https://youtu.be/zPeY70vsHlk I seeded 1000 cells (HeLa) per well and incubated them with fresh medium after treatment (e.g. I...