Cell Biology

23 days ago

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...

4 months ago

As a PLATFORM for SYSTEMS BIOLOGY, the open-source project LIFE123 (https://life123.science) offers a growing set of tools to wrestle with the complications of numerical solutions to complex dynamical systems, such as cycles of biochemical reactions. ONLY 1/2 OF THE BATTLE, is to offer functions to set up and simulate webs...

6 months ago

Get into the holiday spirit with this new paper out in Cell on reindeer! Here, the authors investigated a curious observation in reindeer: the velvet on antlers can regenerate following injury, but skin wounds scar similar to in other mammals. The authors set out to understand how and why the wound regeneration varies. Th...

7 months ago

Every poignant dramatic story has an arch-villain. The aging process has many! A well-known one is chronic inflammation - sometimes referred to as "Inflammaging" (the vicious cycle between inflammation and aging.) An often-discussed source of chronic inflammation are senescent cells - but they aren't the only one. The N...

7 months ago

Hello everyone I hope you are fine and doing great. I am a fresh graduate of Microbiology (Food and Nutrition). I want to hunt a postdoc in my research domain (Microbiome, Probiotics, Genomics). As per my research and collected information, the postdoc positions are competitive and the ones with a high number of pertinent ...

8 months ago

Once in a while, a paper gives a feeling of "Wow, at a junction in History!" Such is a recent entry in Nature Cell Biology, which I will liken to a play: Prelude - Telomere (DNA protective caps) shortening : one of the first recognized "hallmarks of aging"... (general background from NIH: https://www.ncbi.nlm.nih.gov/pmc/...

8 months ago

Protoplasm isolation is a recurrent technique that is needed for the transfection of plant tissue culture and the evaluation of transiently expressed genetic vectors. This is a widely used technique in many plant development labs. However further adaptation might require adjustments on either more recalcitrant salt and buff...

9 months ago

Something I love about scientific conferences is being exposed to less widely known - but potentially very important - avenues of research. Case in point: the accumulation of a toxic biomolecule, an oxidized form of cholesterol (7-ketocholesterol : https://pubchem.ncbi.nlm.nih.gov/compound/7-Ketocholesterol ), believed to ...

9 months ago

GOAL : To develop a safe and effective gene therapy vector! So excited and thankful to share these preliminary results. I am sharing this on youtube in more detail! https://youtu.be/twwOipz50WM In S/MAR vector the best promoter/terminator combination to use for gene expression is Ef1A/BGH in HepG2cells. As also demonst...

9 months ago

Mainstream media is having a field day - paywalled news reports on Wall St Journal, etc. Cutting thru the hype, I think a worthwhile general-public report is https://www.reuters.com/lifestyle/science/scientists-find-clues-what-makes-immortal-jellyfish-immortal-2022-08-29/ It leaves out plenty - but the original paper is f...

Dilution spot assay for yeast

Dilution spot assay for yeast

Open-source platform for Systems Bio : "Life123"

Open-source platform for Systems Bio : "Life123"

Reindeer Provide Insight into Mammalian Wound Healing

Reindeer Provide Insight into Mammalian Wound Healing

NLRP3 - Aging and Chronic Inflammation (beyond Senescent Cells)

NLRP3 - Aging and Chronic Inflammation (beyond Senescent Cells)

Hello everyone..! Is it advisable to publish in any preprint repository?

Hello everyone..! Is it advisable to publish in any preprint repository?

Desperately short telomeres? Alternate way to elongate them!

Desperately short telomeres? Alternate way to elongate them!

Protoplasms preparation for monocot plants

Protoplasms preparation for monocot plants

Removing Toxic Biomolecules that Accumulate with Age

Removing Toxic Biomolecules that Accumulate with Age

Right..since I'm not going to publish this data because lack of funds I might as well share! Behaviour of promoters and terminators (S/MAR episomal vectors) in HEPG2 cells!

Right..since I'm not going to publish this data because lack of funds I might as well share! Behaviour of promoters and terminators (S/MAR episomal vectors) in HEPG2 cells!

Details emerge about MECHANISM of the "INDEFINITE" LIFESPAN of a type of jellyfish

Details emerge about MECHANISM of the "INDEFINITE" LIFESPAN of a type of jellyfish