Karissa Milbury
23 days ago
Method

Dilution spot assay for yeast

Dilution spot assay for yeast

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...
Julian West
4 months ago

Open-source platform for Systems Bio : "Life123"

Open-source platform for Systems Bio : "Life123"

As a PLATFORM for SYSTEMS BIOLOGY, the open-source project LIFE123 (https://life123.science) offers a growing set of tools to wrestle with the complications of numerical solutions to complex dynamical systems, such as cycles of biochemical reactions. ONLY 1/2 OF THE BATTLE, is to offer functions to set up and simulate webs...
Jessie MacAlpine
6 months ago

Reindeer Provide Insight into Mammalian Wound Healing

Julian West
7 months ago

NLRP3 - Aging and Chronic Inflammation (beyond Senescent Cells)

NLRP3 - Aging and Chronic Inflammation (beyond Senescent Cells)

Every poignant dramatic story has an arch-villain. The aging process has many! A well-known one is chronic inflammation - sometimes referred to as "Inflammaging" (the vicious cycle between inflammation and aging.) An often-discussed source of chronic inflammation are senescent cells - but they aren't the only one. The N...
LongevityCell BiologyPharmacologyInflammasomes IL-1β IL-18 Life123 CANTOS BioAge Labs Inflammation Inflammaging NLRP3 Pyroptosis Healthspan

Hello everyone..! Is it advisable to publish in any preprint repository?

Julian West
8 months ago

Desperately short telomeres? Alternate way to elongate them!

Desperately short telomeres? Alternate way to elongate them!

Once in a while, a paper gives a feeling of "Wow, at a junction in History!" Such is a recent entry in Nature Cell Biology, which I will liken to a play: Prelude - Telomere (DNA protective caps) shortening : one of the first recognized "hallmarks of aging"... (general background from NIH: https://www.ncbi.nlm.nih.gov/pmc/...
David Jaime Castillo
8 months ago
Method

Protoplasms preparation for monocot plants

Protoplasms preparation for monocot plants

Protoplasm isolation is a recurrent technique that is needed for the transfection of plant tissue culture and the evaluation of transiently expressed genetic vectors. This is a widely used technique in many plant development labs. However further adaptation might require adjustments on either more recalcitrant salt and buff...
Julian West
9 months ago

Removing Toxic Biomolecules that Accumulate with Age

Removing Toxic Biomolecules that Accumulate with Age

Something I love about scientific conferences is being exposed to less widely known - but potentially very important - avenues of research. Case in point: the accumulation of a toxic biomolecule, an oxidized form of cholesterol (7-ketocholesterol : https://pubchem.ncbi.nlm.nih.gov/compound/7-Ketocholesterol ), believed to ...
LongevityCell BiologyPharmacologyCholesterol Atherosclerosis Cyclodextrins 7-ketocholesterol Cyclarity Therapeutics Matthew O'Connor Conferences Ending Age-Related Diseases 2022 UDP-003 Candymer software Healthspan
Adrian Ionut Pascu
9 months ago

Right..since I'm not going to publish this data because lack of funds I might as well share! Behaviour of promoters and terminators (S/MAR episomal vectors) in HEPG2 cells!

Right..since I'm not going to publish this data because lack of funds I might as well share! Behaviour of promoters and terminators (S/MAR episomal vectors) in HEPG2 cells!

GOAL : To develop a safe and effective gene therapy vector! So excited and thankful to share these preliminary results. I am sharing this on youtube in more detail! https://youtu.be/twwOipz50WM In S/MAR vector the best promoter/terminator combination to use for gene expression is Ef1A/BGH in HepG2cells. As also demonst...
Julian West
9 months ago

Details emerge about MECHANISM of the "INDEFINITE" LIFESPAN of a type of jellyfish