Mandy Lorewall
15 days ago
Question

Cell Culture Question:

Adán Andrés Ramírez Rojas
2 months ago
Pro Lab Tip

"Mid/High-throughput" transformation

Nick Gervais
9 months ago
Method

'You vs. the Guy She Tells You Not to Worry About': Heat-Shock vs. Electroporation

'You vs. the Guy She Tells You Not to Worry About': Heat-Shock vs. Electroporation

I compared the results from identical cloning protocols preceding either a heat-shock or electroporation bacterial transformation into NEB competent E. coli cells (chemically competent or electrocompetent) and found that the electroporation transformation consistently resulted in thousands more colonies than the heat-shock ...