Karissa Milbury
21 days ago
Method

Dilution spot assay for yeast

Dilution spot assay for yeast

This is a quick run-through of the protocol to make dilution spot plates for S. cerevisiae. This assay is great for identifying broad fitness defects but isn't quantitative. Note that temperatures for temperature sensitive strains will need adjustment from the standard 30°C. Even if you aren't aware of any temperature-rela...

Why my KB Agar went cloudy and why you should check your growth medium reagents.

Why my KB Agar went cloudy and why you should check your growth medium reagents.

I want to talk about preparing basic microbiological growth media and things that can go wrong when swapping reagents. Weird cloudy KB Agar A while ago, I was preparing KB Agar: Recipe for 1L KB Agar 10g Glycerol 20g Peptone 1.5g K2HPO4 trihydrate 12g Agar powder 5 mL sterile 30% MgSO4 solution (to be added after autocla...
MicrobiologyBacteria Soil isolates KB Agar Growth MediumReagentsAgar Medium Variovorax spp. Ochrobactrum spp. Alcaligenes spp./Achromobacter spp. Stenotrophomonas spp.