Hi all,

I have been using this very very quick gDNA extraction (<5 min) method for plants for a few years now and wanna share it here in case you find it useful. I mainly use it for Mimulus, Aquilegia, but tried it in Arabidopsis too, most likely applies to many other plants:

1. collect the tip (~ 1cm for small leaves, ~2cm for larger leaves) of the leaf into 2.0 tube
2. add 60 ul NaOH(0.4m) and grind it
3. incubate at 60C for 1 min
4. spin max speed for 1min
5. take 1ul supernatant into 49ul tris(0.1m, ph=8; if you feel you won't get too much gDNA just lower the volume of tris)
6. use 1-1.5 to PCR 
