Resuspending Twist Oligo Pools?
I've received a custom oligo pool from Twist Bioscience and they provided 70ng of it. We talked to one of the reps and they said not to resuspend it to any less than 10ng/μL, which means I'll be adding less than 7μL of buffer, which is obviously not a lot. I was wondering if anyone had any practical tips for how to make this work and not lose any DNA. Is 10ng/μL really the lowest you can safely store DNA at? What centrifugation time/speed do you use to spin it down first? Do you heat it at all before reuspending? Do you vortex or just pipette up and down to mix? Any advice would help! Thank you.
Seems like an odd proposition considering that DNA is highly stable. Do you know how much of these oligos you need for downstream reactions? 7uL does seem like a small volume and you can run the risk of it evaporating (and thus increasing the remaining concentration) or just overusing it.