Henrik Ellinghaus
posted 8 months ago

PCR (Polymerase Chain Reaction)

PCR is likely one of the most useful techniques in biology labs. It was invented by Kary Mullis in 1984. Nowadays it helps us especially if DNA material needs to be amplified to work with higher quantities, but the applications of this method are almost endless.

Each PCR protocol uses the same standard ingredients. The protocols mainly differ in minor things such as the type of Polymerase used or the Buffer (and which concentration of salts it contains). Also annealing temperatures vary depending on the length and GC content of the sequence to be amplified. Feel free to share your PCR protocols (ingredients) down here...

Henrik Ellinghaus8 months ago

My protocol (used for cloning PCR): 10 µl 5X Prime START GXL buffer, 4 µl dNTPs (2.5 mM), 0.25 µl Sense primer (100 µM), 0.25 µl Antisense primer (100 µM), 0.3 µl Prime START GXL DNA polymerase, 5 µl Template DNA (~100 ng), 30.2 µl ddH2O (Water)

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