Adán Andrés Ramírez Rojas
posted 2 months ago
(he/they) Colombian PhD. working in synthetic genomics. Interests: NGS, genome editing in yeast, synthetic chromosomes, lab automation. Out of the lab: diversity and inclusivity in research and academia. Representation matters.
Pro Lab Tip

"Mid/High-throughput" transformation

I always found it annoying when doing several transformations in E. coli having to use a lot of agar plates for it. I'm not a huge fan of over-stacking plates, and then when doing manual colony picking for screening having too many plates is not my favorite thing. Well, in my current lab, I was given this trick to use when performing a lot of transformations of relatively simple constructs (e.g. golden gates of a single fragment into a vector). The only thing you need is to use square (120mm) or SBS plates for pouring your media. Then, after your recovery step (if performed) in the transformation, put the plate in an inclined position (I literally use a box of tips to use as support), and depending on the expected efficiency of the transformation, put a drop of 10-20 µL on the top of the plate and let it slide down. In this way, you can use a single plate for 8-12 transformations.
I don't know how popular this is, but I never saw this kind of trick when working in other labs, and when we have a new person here, they seem pretty excited about trying out themselves. P.S. If you're performing the transformation via heat-shock, you can work from the beginning in deep well plates, and in every following step in which pipetting is required, just using a multi-channel. Let me know if you would like me to post a detailed protocol at some point :)

Keith Loritz2 months ago

Thanks Adán! . . . if I could do a 20 channel wide version of this . . . :-)

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