Has anyone ever optimized/troubleshot a NASBA RNA amp before?
I have a mentor whose company is struggling to optimize a NASBA reaction. The reaction does amplify the target, but it takes over an hour, and the target yield is low, with lots of non-specific amp.
If anyone has any ideas on how to optimize, this would be greatly appreciated!
although haven't done much of these I can give my two cents on amplification experiences in general (from working with RNA derived libraries/genomic based applications).