Has anyone ever optimized/troubleshot a NASBA RNA amp before?

although haven't done much of these I can give my two cents on amplification experiences in general (from working with RNA derived libraries/genomic based applications).

1. firstly there is just the basics, MgCl, pH, other buffers (although to be honest I didn't find these to be the culprits usually)
2. primer design -- the non-specific amplification and low yield would indicate that to me. Really double, triple, quadruple check that...that was usually the problem.
3. RNA quality? it could be degraded, but I assume that was checked at the onset
4. enzyme -> expiration date...I know it's obvious but I have made that mistake too~
5. blast it with more primers, more input, more enzyme

This is awesome @Guy, thank you! I will pass along.