Custom-made CRISPR pooled gRNA KO library_Lentiviral vectors
I want to use a CRISPR library to knockout a certain number of human genes that I'm interested in.
Since I'm new in the field of genome editing, I would like to find a reliable company to generate for me the library in the form of ready-to-transduce lentiviral-vectors.
I am going to follow the Nature protocols 2017 of Joung et al. that I found really helpful, so I would like the library to be made either in lentiCRISPRv2 or lentiGuide-puro vector backbone.
My questions are:
- Can any of you recommend or had experience with a company that can provide custom-made libraries in a customer-provider vector backbone in the form of lentiviral vectors?
- In order for the Nature protocols 2017 of Joung et al., to be applied in a custom-made library what factors should I have in mind before ordering the library?
Please check Dharmacon. They have whole range of custom-made library.