David Walker-Sünderhauf
posted 4 months ago

Any broad comparisons of promoter strengths?

Hi folks,

I found a nice overview over the strength of various BioBricks promoters https://jbioleng.biomedcentral.com/articles/10.1186/1754-1611-3-4

I'm especially interested in finding a constitutive promoter (family) so I can test a range of promoters with different known strengths, for example the BioBricks J23100 promoter family. http://parts.igem.org/Part:BBa_J23119

However, I'm struggling to find a benchmark for this. Currently, I'm using the P3 bla promoter (widely used e.g. on pBR322 plasmids) in the same system, but this is too strong. Does anybody happen to know whether a broader comparison of commonly used promoters exists?

Adrian Ionut Pascu4 months ago

This is what I did. Check the results section (the graphs). They are mammalian promoters btw (commonly used ones). Hek293T, Hela and HepG2 seem to perform similar. Figures 8,10,11,12. https://www.future-science.com/doi/10.2144/btn-2022-0041

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